Twenty-two patients in Vietnam have contracted severe infections and some may suffer permanent sight loss due to contamination of a chemical used during surgery, officials said Thursday, according to dpa. Some of the affected patients at the Ho Chi Minh City Eye Hospital may need to have one or both eyes removed, said senior Ministry of Health official Ly Ngoc Kinh. One of the patients had already had an eye removed following the infection, according to one news report. Another report said that two patients were likely to go blind as a result. Doctors have blamed the infections on contamination of a batch of the tissue-staining agent Trypan Blue, manufactured by the Indian pharmaceutical company Khosla Medical & Surgical, Inc. The agent is used to highlight eye structure during surgery. Ministry of Health tests confirmed that bottles from a batch of Trypan Blue were contaminated with the blue pus bacillus, psuedomonas aeruginosa. Representatives of the Viet My Mechanical Equipment Company, which imported the drug, refused to comment. The patients developed bruises and pus in their eyes last month following surgery at the hospital. Hospital officials have asked Khosla to acknowledge responsibility and compensate the victims, but the company has yet to respond. Health officials said they had recalled Khosla's Trypan Blue from health institutions around the country.